Mark Wefers Bettink

Chapter 5 92 Results Experiment A Hemodynamic parameters Table 1 presents data on hemodynamic parameters of experiment A. At baseline (T0) all rats were hemodynamically stable and no significant difference was observed between the groups. Three hours after LPS infusion heart rate (p=0.0293) and lactate (p=0.0007) increased compared to the time-control group. MitoPO 2 measurements In accordance with previous experiments (Harms et al., 2015a), the application of ALA increased the amount of PpIX in the mitochondria to detectable levels in all animals. MitoPO 2 could be measured in the abdominal rat skin for the duration of the experiment (3 hours after application of ALA). MitoPO 2 in the abdominal skin didn’t change for 3 hours (T0  T1) in both the time control (M- TC) and endotoxemia (M-LPS) group (Fig 2A). However, muscle mitoPO 2 at T1 in the M-TC group was markedly higher, 82 [42] mmHg vs 46 [27] mmHg, compared to the M-LPS group (Fig 2B).