Mark Wefers Bettink

Monitoring mitochondrial respiration 5 95 Experiment B Hemodynamic parameters At baseline (T0) all rats were hemodynamically stable and no significant difference was observed between the groups, as shown in table 2. Three hours after LPS infusion (T1), significant hemodynamic changes were observed in the LPS groups, reflected by an elevation in serum lactate levels in all three endotoxemia groups. A decrease in mean arterial blood pressure (MAP) was seen in the LPS -- and the LPS ++ group compared to TC, but not in the LPS + - group. Cardiac output decreased in the LPS -- (p=0.049) and LPS ++ (p=0.039) groups (102 ± 59 and 100 ± 39 bpm vs 140 ± 56 bpm TC) compared to time control. The succinate control group showed a significant decrease in heart rate (p=0.0245), 267±11 vs 290 ±13 bpm, compared to time control. MitoPO 2 measurements A mitoPO 2 value around 60 mmHg was observed for all experimental groups at T0. In the LPS -- group a significant decline in mitoPO 2 from 62 ± 9 mmHg (T0) to 41 ± 12 mmHg (T1) was measured. At T1 a significant lower value of mitoPO 2 was measured in the LPS -- and LPS +- (p=0.0006) groups compared to time control, of 41 ± 12 mmHg (LPS --) and 54 ± 21 mmHg (LPS +-) compared to 74 ± 17 mmHg (TC) respectively (Fig. 4A). The individual changes in mitoPO 2 are presented in Figure 4B.