Mark Wefers Bettink

Chapter 5 96 T0 T1 0.0 0.5 1.0 1.5 2.0 LPS - - mitoPO 2 (normalized) T0 T1 0.0 0.5 1.0 1.5 2.0 LPS + - mitoPO 2 (normalized) T0 T1 0.0 0.5 1.0 1.5 2.0 LPS + + mitoPO 2 (normalized) Mitochondrial PO 2 T0 T1 0 50 100 150 LPS - - LPS + - LPS + + * # Time control Succinate MitoPO 2 (mmHg) # T0 T1 0.0 0.5 1.0 1.5 2.0 TC mitoPO 2 (normalized) T0 T1 0.0 0.5 1.0 1.5 2.0 SC mitoPO 2 (normalized) Normalized individual mitochondrial PO 2 A B Figure 4. Panel A: MitoPO 2 at T0 and T1 in the different experimental groups. Panel B: Percentage change in individual mitoPO 2. TC; time control, SC; methyl-succinate control, LPS --; endotoxemia, LPS +-; endotoxemia with fluid resuscitation, LPS ++; endotoxemia with fluid resuscitation and methyl-succinate administration, LPS; lipopolysaccharide, mitoPO 2 ; mitochondrial oxygen tension. T0 and T1 are the time points of the mitoPO 2 measurement. Data are presented as mean ± SD, * significant difference compared to baseline measurement, # significant difference compared to time control measurement (p<0.05); (8 rats/group). LPS -- T1 vs LPS -- T0 (p=0.0015), LPS -- T1 vs TC T1 (p<0.0001) and LPS +- T1 vs TC T1 (p=0.0006). ODR measurements The ODR measurements at baseline (T0) did not differ significantly between the groups. In three groups ODR at T1 decreased compared to the baseline measurement (T0); the succinate control decreased from 4.6 ± 1.2 mmHg*s -1 to 3.6 ± 0.5 mmHg*s -1 , the LPS -- group decreased from 4.5 ± 1.0 mmHg*s -1 to 2.4 ± 1.6 mmHg*s -1 , and the LPS +- group decreased from 4.2 ± 1.3 mmHg*s -1 to 3.1 ± 1.0 mmHg*s - . However, infusion of methyl-succinate maintained ODR in the endotoxemia group (LPS++) at baseline values (Fig. 5).