15353-j-veluchamy

102 | Chapter 4 Figure 4: Combinatorial effect of NK cells and cetuximab on EGFR +/- , RAS wt / mut and BRAF mut cells Cytotoxicity effected by cetuximab and NK cells either alone or in combination against COLO320 (EGFR - , RAS wt ), Caco-2 (EGFR + , RAS wt ), SW480 (EGFR + , RAS mut) , SW620 (EGFR + , RAS mut) and HT-29 (EGFR + , RAS wt, BRAF mut) , was compared between these cell lines measuring target cell death (A). Functional differences between NK cells that were co-cultured with tumor cell lines in the presence or absence of cetuximab were evaluated by studying NK cell degranulation (B). Data presented is from five individual PBNK donors. Columns are mean of triplicate values from five experiments; bars represent SD. Mean ± SD for each significant condition are represented as p = <0.05 *, <0.01 **, <0.005 ***, <0.001 ****.