15353-j-veluchamy

4 NK cell ADCC enhances treatment efficacy in colorectal cancer | 103 Figure 5: Cetuximab enhances NK cell killing of primary colon tumors through ADCC Primary colon tumor cells were pretreated with cetuximab and incubated with activated NK cells to monitor ADCC effects. NK cells and cetuximab alone controls were included to document the effects of monotherapy. Percentages of tumor cell death from five CRC patients are shown in figure A and each symbol represents one specific patient. RAS wt tumors I, II and III are indicated by open squares, triangle and circles, and RAS mut tumors IV and V are shown as closed rhombus and hexagon. Figure B shows only the cytotoxicity data of RAS wt tumors. All five primary tumors used in the cytotoxicity assay were tested for EGFR and HLA-ABC expression as shown in figures C and D. Grey histograms represent the isotype controls, black shades represent EGFR and HLA-ABC expression for tumor samples I-V. Data points from figures A and B are mean of triplicate values from five patients; each significant condition are represented as p = <0.05 *, <0.01 **, <0.005 ***, <0.001 ****.