15353-j-veluchamy

4 NK cell ADCC enhances treatment efficacy in colorectal cancer | 115 Supplementary Figure 1: Cetuximab cytotoxic activity against EGFR + RAS mut and EGFR - RAS wt colon cancer cells  SW480 and COLO320 cells were incubated with 100µg/ml of biotinylated cetuximab for 1hr at 4°C and analyzed for their EGFR recognition by cetuximab. A and B, Histograms showing binding of cetuximab to (A) COLO320 and (B) SW480 cells. Grey shades represent the streptavidin APC control, black shades represent binding of cetuximab. Further, SW480 and COLO320 cells were stained with PBSE and exposed to increasing concentrations of cetuximab for 1hr at 4°C after which unbound antibodies were removed and cells were cultured for an additional 4hrs at 37°C. Target cell death was determined by assessing the percentage of 7AAD positive COLO320 (C) and SW480 (D) cells at the end of incubation.