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116 | Chapter 4 Supplementary Figure 2: Degranulation of NK cell CD56 bright and CD56 dim subsets in response to cetuximab coated tumor target cells A representative example of PBNK degranulation pattern upon target exposure to A431 tumor targets in the presence or absence of cetuximab. CD56 bright and CD56 dim degranulate upon target cell recognition (Figure A), with an increase in degranulation in CD56 dim CD16 + subset of NK cells when target cells are coated with cetuximab (Figure B). The degranulation can be reduced by blocking Fc receptors (Figure C) and this also decreases the degranulation in the CD56 bright subset of NK cells. Supplementary figure 3: Expression of HLA-E on tumor cell lines and NKG2A on NK cells used for cytotoxicity experiments Surface expression of HLA-E on COLO320, Caco-2, SW620, SW480 and HT-29 and NK cell NKG2A expression levels of five healthy donors used for the cytotoxicity assays were determined by flow cytometry as shown in figure A and B. Columns are mean of triplicate values from two independent experiments, bars represent SD. Mean ± SD for each significant condition are represented as p = <0.05 *, <0.01 **, <0.005 ***, <0.001 ****.