15353-j-veluchamy

44 | Chapter 2 Table 4: Comparison between instruments of the increment in average cell frequencies obtained after staining with the panels components. Reference sample I Reference sample II Reference sample III Antibodies Canto Fortessa MQ p- value Canto Fortessa MQ p- value Canto Fortessa MQ p- value CD45 VioGreen 98.3 97.2 98.3 ns 99.5 94.0 98.5 ns 97.0 97.0 99.0 ns CD3 VioBlue 75.6 73.0 76.7 ns 75.3 73.4 77.5 ns 67.2 69.0 71.7 ns CD14 VioBlue 1.13 2.5 1.0 ns 1.11 3.7 1.0 ns 1.7 12.92 1.1 ** CD19 VioBlue 16.0 16.4 14.5 ns 7.7 7.7 12.5 ns 4.19 9.76 20.2 * Sytox Blue 17.0 12.4 18.5 ns 16.2 13.1 14.5 ns 13.6 12.3 19.0 ns CD56 APC-Vio770 2.9 2.5 2.4 ns 2.2 3.0 2.7 ns 9.29 9.8 8.3 ns CD3 PerCP-Vio700 76.3 74.0 77.5 ns 74.6 77.5 77.4 ns 68.4 69.7 72.1 ns TCRγδ PerCP-Vio700 1.9 2.5 2.9 ns 1.7 2.7 3.23 ns 2.6 2.5 2.9 ns TCRγδ VioBlue 1.6 1.6 2.4 ns 1.7 1.4 2.71 ns 2.5 2.7 2.4 ns CD56 + CD16 APC 33.0 28.0 31.6 ns 33.4 30.0 33.7 ns 36.0 34.5 31.5 ns CD56 + NKG2A PE-Vio770 59.0 63.4 57.5 ns 59.6 63.8 61.0 ns 48.8 51.6 62.9 ns CD56 + NKG2C PE 6.3 6.2 6.4 ns 7.9 4.9 6.0 ns 11.0 10.7 12.7 ns CD56 + NKG2D PerCP-Cy5.5 92.6 91.3 92.9 ns 90.8 94.9 92.8 ns 97.8 95.5 93.5 ns CD56 + PanKIR2D FITC 35.1 33.9 36.0 ns 34.2 33.9 29.2 ns 18.1 19.3 21.4 ns CD56 + CD25 VioBrightFITC 7.2 7.8 8.0 ns 6.9 7.2 7.4 ns 22.3 23.4 25.2 ns CD56 + NKp44 PE-Vio770 1.0 0.8 1.2 ns 5.6 4.4 6.6 ns 6.1 6.0 7.5 ns CD56 + CD107a PE 23.7 24.2 19.5 ns 24.0 25.2 26.3 ns 31.2 28.2 33.8 ns Canto: BD FACSCanto TM II; Fortessa: BD LSRFortessa TM ; MQ: MACS Quant Analyzer® X; p-value (Kruskal Wallis test) *p=0.03, **p=0.001.