15353-j-veluchamy

2 Harmonized NK FACS panels to study NK cell phenotype and function | 47 Figure 4: Experimental design to compare different PBMC conditions The experimental set up describes the work flow to compare NK phenotype and function changes under different PBMC (non-stimulated and stimulated) conditions. PBMC from 12 healthy donors were stained with NK cell phenotype and NK function panel cocktails. Data was acquired for 6 donors using BD LSRFortessa TM and for other 6 donors in MACSQuant ® Analyzer X. Further, FCS files from LSRFortessa TM were analysed on KALUZA ® and for MACSQuant ® Analyzer X in MACSQuantify TM 2.8. Data obtained from both devices were combined and examined for statistical significance between fresh and cryopreserved NK cells.