15353-j-veluchamy

2 Harmonized NK FACS panels to study NK cell phenotype and function | 49 Figure 6: Comparison of NK cell function between fresh and cryopreserved NK cells NK cell functions between freshly isolated and cryopreserved thawed PBMC samples were compared. NK cells were either activated or non-activated with cytokines (IL-2+IL-15) and target cells (A431) alone or targets coated with cetuximab (CET). Expression levels of CD107a (A), CD16 (B), NKp44 (C), CD25 (D) were compared for the following conditions: i) NK only ii) NK + A431 and iii) NK + A431 + CET conditions. NK only conditions are depicted as open rectangles, followed by NK + A431 with grey shades and NK + A431 + CET conditions represented as black rectangles. Columns represent data from12 donors, from each donor the mean of triplicate values was used; with bars showing SEM. Data are from independent experiments performed in triplicates from 12 PBMC donors (6 donors: BD LSRFortessa + 6 donors: MACSQuant). Statistical analysis was performed using the Wilcoxon test. Table 5: Comparison of NK phenotype and function parameters between non-activated fresh and cryopreserved samples. Non-activated fresh versus cryopreserved NK samples (Wilcoxon test) NK FACS panel drop ins NK only NK + A431 NK + A431 + CET CD56 + NKG2A PE-Vio770 ns ns ns CD56 + NKG2C PE ns ns ns CD56 + NKG2D PerCP-Cy5.5 ns ns ns