15353-j-veluchamy

50 | Chapter 2 CD56 + PanKIR2D FITC ns ns ns CD56 + CD107a PE ns ns ns CD56 + CD16 APC ns ns ns CD56 + NKp44 PE Vio770 ns ns ns CD56 + CD25 VioBrightFITC ns ns ns CD56 dim CD16 APC ns ns ns Non-activated freshly isolated NK cell samples from 12 healthy donors and their corresponding cryopreserved counterparts were stained with the NK phenotype and NK function antibody mix and compared under different stimulation conditions (NK only, NK + A431, NK + A431 + CET). All samples were measured in triplicates. Statistical analysis to detect differences between non-activated fresh and cryopreserved samples was performed using the Wilcoxon test. No significant p-values (<0.05) were obtained for the assessed NK phenotype and function panel drop-in parameters. Marker expression level of individual donors are shown in supplementary figures S4, S5 and S8B online. Table 6: Comparison of NK phenotype and function parameters between cytokine activated fresh and cryopreserved samples. Activated fresh versus cryopreserved NK samples (Wilcoxon test) NK FACS panel drop ins NK only NK + A431 NK + A431 + CET CD56 + NKG2A PE-Vio770 ns ns ns CD56 + NKG2C PE ns ns ns CD56 + NKG2D PerCP-Cy5.5 ns ns ns CD56 + PanKIR2D FITC ns ns ns CD56 + CD107a PE ns ns p=0.06 ns CD56 + CD16 APC ns ***p=0.0010 ns CD56 + NKp44 PE Vio770 ns ns ns CD56 + CD25 VioBrightFITC ns * p=0.03 ns CD56 dim CD16 APC ns ***p=0.0010 ns Cytokine activated freshly isolated NK cell samples from 12 healthy donors and their corresponding cryopreserved counterparts were stained with the NK phenotype and NK function antibody mix and compared under different stimulation conditions (NK only, NK + A431, NK + A431 + CET). All samples were measured in triplicates. Statistical analysis to detect differences between non-activated fresh and cryopreserved samples was performed using the Wilcoxon test. No significant p-values (<0.05) were obtained for the assessed NK phenotype and function panel drop-in parameters. Marker expression level of individual donors are shown in supplementary figures S6 and S7 and S8C online. Non-activated fresh versus cryopreserved NK samples (Wilcoxon test)