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58 | Chapter 2 population (CD45+CD3-/TCRγδ- CD56+) was used to analyse the expression of CD107a, CD25 and NKp44 (Supplementary Fig.S3 online). Statistical analysis Statistical analysis was performed using GraphPad Prism software. Differences between centers were determined using Kruskal Wallis test and difference between fresh and cryopreserved were determined using non-parametric Wilcoxon test. P value of < 0.05 was considered statistically significant. Ethics Statement The research presented in this paper, all methods and experimental protocols were approved and carried out in accordance with relevant guidelines and regulations of the Committee for Scientific Research of the VU University Medical Center, Cancer Center Amsterdam. Acknowledgements This study was supported by the European Research Council, under the NATURIMMUN consortium (ITN FP7-PEOPLE-2012-ITN-317013). The authors like to thank all the healthy human blood donors that participated in this study.