15353-j-veluchamy

2 Harmonized NK FACS panels to study NK cell phenotype and function | 63 Supplementary figure 1: Fluorescence minus one (FMO) controls for gating NK function panel receptors In case of NK cell function panel experiments, a Fluorescence Minus One (FMO) control was devised to optimize the identification of NK cell activation antigens CD25, NKp44 and CD107a under different stimulated conditions. FMO stains and a fully stained sample of NK cell stimulated with cetuximab coated A431 tumor target cells is shown in figure A and B. The FMO gate clearly indicates the negative control position.