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64 | Chapter 2 Supplementary figure 2: Gating strategy for the NK cell phenotype panel The gating procedure to analyse NK cell phenotypes from PBMC population is shown. Cells acquired were gated FSC-H/FSC-A to eliminate doublets, followed by gating on the exclusion channel (CD3/ TCRγδ/CD14/CD19/SYTOX® Blue) against CD45. Lymphocytes negative for exclusion channel antigens and positive for CD45 were then plotted for CD45+CD56+ cells. CD56+ NK cells were then gated to define single receptor positive cells CD56+CD16+, CD56+NKG2A+, CD56+NKG2C+, CD56+NKG2D+, CD56+KIR2D+ plots from CD56+NK cells. Further to facilitate NK subset analysis, NK cells were sub- divided into NK bright and NK dim populations based on CD56 expression and CD16 levels. CD45+CD56- cells can also be studied using this gating strategy.