15353-j-veluchamy

2 Harmonized NK FACS panels to study NK cell phenotype and function | 67 Supplementary figure 5: Comparison of NK function panel marker differences between fresh and cryopreserved non-activated NK cells The capacity of NK cells to perform natural cytotoxicity and ADCC under different NK stimulated conditions were analysed on 12 healthy donors using the NK function panel antibody mix. Expression levels of CD107a (A), CD16 (B) NKp44 (C) and CD25 (D) were compared between fresh and cryopreserved non-activated NK cells for the following conditions: i) NK only ii) NK + A431 and iii) NK + A431 + CET. Data points represent the mean of triplicate values from independent experiments from 12 PBMC donors (6 donors: BD LSRFortessa + 6 donors: MACSQuant). Statistical analysis was done using Wilcoxon test and no statistically significant differences were observed between fresh and cryopreserved non-activated NK cells.