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68 | Chapter 2 Supplementary figure 6: Comparison of NK phenotype panel marker differences between fresh and cryopreserved cytokine activated NK cells NK cells were activated overnight with IL-2 and IL-15 and stimulated with target cells (A431) alone or targets coated with cetuximab (CET). Expression levels of NKG2A (A), NKG2C (B), NKG2D (C), KIR2D (D) were compared between fresh and cryopreserved NK cells for the following conditions: i) NK only ii) NK + A431 and iii) NK + A431 + CET. Data points represent the mean of triplicate values from independent experiments from 12 PBMC donors (6 donors: BD LSRFortessa + 6 donors: MACSQuant). Statistical analysis was done using Wilcoxon test and no statistically significant differences were observed between fresh and cryopreserved cytokine activated NK cells.