15353-j-veluchamy

3 HLA independent killing of cervical tumors by UCB-NK cells | 77 Next, activated PBNK were compared with UCB-NK for their ability to induce target cell death. UCB-NK were significantly more cytotoxic than PBNK, consistently inducing higher rates of tumor cell death in all tested cell lines (P < 0.001) (Figure 2a, b). Note that the PBNK cytotoxicity data presented in Figure 2a are the same as those in Figure 1a. Indeed, the cytotoxicity levels were similar for UCB-NK and PBNK + CET (Figure 1a, 2a). This was Figure 1: PBNK cytotoxicity against cervical cancer cells alone and in combination with CET (a) Cytotoxicity levels (∆7AAD) of activated PBNK (open bars) and PBNK + cetuximab (CET) (closed bars) against ten cervical cancer cell lines, (b) arranged in order of EGFR expression level. Bars are means of triplicate values from four experiments with four different donors for C33A, HeLa, SiHa, CC11B, CC11A, CC10B, CC10A, CaSki and two experiments with two different donors for CSCC7 and CC8. Bars represent mean ± SEM. (c) Significantly higher cytotoxicity levels (∆7AAD) were observed in all cell lines after co- culture with PBNK + CET compared to PBNK, except for C33A (open circle). * P <0.05 and **P < 0.01 calculated with paired t test.