15353-j-veluchamy

3 HLA independent killing of cervical tumors by UCB-NK cells | 81 Figure 4: Effects of NK inhibitory ligands on NK cytotoxicity against cervical cancer cells (a) Percentage of positive cells within the NK cell population for NK inhibitory receptors KIR2D and CD94/ NKG2A for PBNK only, PBNK stimulated with cytokines (IL-2+IL-15) and UCB-NK only were determined by flow cytometry. The data presented is from three representative donors for PBNK and UCB-NK. PBNK only are denoted by open circles, PBNK (IL-2+IL-15) are denoted by closed circles and UCB-NK by closed squares. (b) Representative histogram plots showing geometric mean fluorescence intensity (MFI) of NK inhibitory ligands HLA-ABC, HLA-E and HLA-G on cervical cancer cells; representative plots of 2-3 separate analyses are shown. Correlation analysis of MFI of HLA-ABC with % cytotoxicity (∆7AAD) by (c) PBNK, (d) PBNK + cetuximab (CET), and (e) UCB-NK. Dotted lines represent 95% confidence interval of the regression line. Four experiments with four different PBNK donors for C33A, HeLa, SiHa, CC11B, CC11A, CC10B, CC10A, CaSki, two experiments with two different PBNK donors for CSCC7 and CC8, and five experiments with five different UCB-NK donors were used for this experiment. P-value was calculated with Pearson analysis.