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92 | Chapter 3 Supplementary Figure 1: Representative example of flowcytometric analysis A representative example of flowcytometric analysis of Natural Killer (NK) cytotoxicity assay with cell line CC10A. (a) Identification of effectors and targets using SSC and FSC gating. (b) Target cells stained with PBSE were gated out from effector cells and cell debris. (c) PBSE-positive target cells were then gated against 7AAD to determine the percentage of cell death induced by NK cells. (d) Cells staining negative for PBSE and low on SSC were gated against CD56 marker to identify NK cells, further on NK cells, the levels of (e) NK degranulation (CD107a) and (f) NK CD16 + cells percentages were calculated and compared with respective target only controls.