15353-j-veluchamy

3 HLA independent killing of cervical tumors by UCB-NK cells | 93 Supplementary Figure 2: NK degranulation levels per cell line for PBNK, PBNK + CET, and UCB-NK Significantly higher levels of NK degranulation (ΔCD107a) in PBNK + cetuximab (CET) and UCB-NK conditions compared to PBNK only condition for SiHa, CC10A and CaSki. Bars are means of triplicate values from four experiments with four different donors for C33A, HeLa, SiHa, CC11 B, CC11A CC10B, CC10A, CaSki and two experiments with two different donors for CSCC7 and CC8 using PBNK and five experiments for UCB-NK using five different donors for all cell lines. *P <0.05 and **P 0.01 calculated with one-way ANOVA, Bonferroni’s multiple comparison test. Supplementary Figure 3: Cytotoxicity levels per histological subtype and HPV type PBNK (open bars), PBNK + cetuximab (CET) (closed bars), and UCB-NK (hatched bars) cytotoxicity levels per (a) histological subtype and (b) HPV type of cervical cancer cell lines. Bars represent mean ± SEM. Four experiments with four different PBNK donors for C33A, HeLa, SiHa, CC11B, CC11A, CC10B, CC10A, CaSki, two experiments with two different PBNK donors for CSCC7 and CC8, and five experiments with five different UCB-NK donors were used for this experiment. AC: adenocarcinoma; SCC: squamous cell carcinoma; ASC: adenosquamous cell carcinoma.