Suzanne de Bruijn

198 Chapter 4 Table S2. Primer sequences utilized to validate and characterize breakpoints SV Breakpoint Coordinates F primer (5’-3’) R primer (5’-3’) Amplicon size (bp) NL-SV1 A-B 57,291,905 GCCTGGGTGACTAAGAAAGACTCCATTCCC CCACGGAGCACCTTGTAGCTCATTAACTGC 720 B-B 57,518,137-57,291,905 GGCACTAATGAAACCAGAAAGACACTTGGC CCACGGAGCACCTTGTAGCTCATTAACTGC 839 B-C 57,518,137 GGCACTAATGAAACCAGAAAGACACTTGGC TAGTCATAGTCCCTGATTCCCTTAAAGCGG 831 UK-SV2 D-E 57,275,839 CATGACAAAACCCTGTCTCC CCTATCCAGTAAATGCCTCTTCC 881 E-[G] 57,456,098-57,559,114 ATCAGGCAACACGACACCAT AGAGTGTTAACAAAGTAGACTCGAT 1262 [G]-[F] 57,468,960 GGAGCCTGAAGGAGTTGTCAAA AATCCAACACATCTTCAGGGCA 999 [F]-[E] 57,456,098 ATCAGGCAACACGACACCAT TCTTCCACATGGGGACATAGG 894 [E]-G 57,275,839-57,468,960 CCTATCCAGTAAATGCCTCTTCC AATCCAACACATCTTCAGGGCA 1427 G-H 57,559,114 AGAGTGTTAACAAAGTAGACTCGAT ACTGGCCAAAGAAAGACCCT 989 SA-SV3 I-J 57,247,615 GGGTGCAGTCATTCATTCCT TCTCTTGAGCCCAGGAAATC 513 J-K 57,391,678 TCATGTGAAATGCCACCTTC GAGTGTAACGGCATGGTCTC 1530 K-L 57, 499,214 TTCTTTTAAGGGGGACCTTG AAGCCAAGATCATCCAAACC 694 L-J 57,516,678-57,247,615 TGCCACTTTCCATATGTGTG TCTCTTGAGCCCAGGAAATC 657 J-[M] 57,391,678-57,612,711 TTATGAATCTGCCCAAGATCAC AATGATTTGCCTTGGCTTTC 1022 [M]-L [57,516,678]-57,499,214 GAATTTGCTTGAAGGGCTTG AAGCCAAGATCATCCAAACC 486 L-M 57,516,678 TGCCACTTTCCATATGTGTG GAATTTGCTTGAAGGGCTTG 702 M-N 57,612,711 AATGATTTGCCTTGGCTTTC CAATGCCATACTCTGGACACC 823 CA-SV4 O-P 57,233,035 GAAGAGCCAACCAATCACAC AACAGGCCCAGCTACTCAAG 368 P-[R] 57,280,008-[57,634,900] ATACAGGGAGACCCCGTTTC CTGATCGAAGTGCAAAATGG 1801 [R]-[Q] 57,483,883 CTACACAGGGGACTGACACC CAGCAGCAGCATTATCAACC 677 [ Q]-[P] 57,280,008 ATACAGGGAGACCCCGTTTC AGATGAGTTCTTGCTCTGTTGC 653 [P]-R [57,233,035]-57,483,883 CAGCAGCAGCATTATCAACC AACAGGCCCAGCTACTCAAG 493 R-S 57,634,900 CTGATCGAAGTGCAAAATGG TGGAGGGAAGGTTATCTTGG 2299