Suzanne de Bruijn

222 Chapter 4 YPEL2 A C Brain Duodenum Heart Kidney Liver Lung Retina Skeletal muscle Spleen Stomach Testis Thymus 0.00 0.02 0.04 0.06 0.08 0.10 0.30 0.35 0.40 0.45 0.50 Relative expression Brain Duodenum Heart Kidney Liver Lung Retina Skeletal muscle Spleen Stomach Testis Thymus 0.00 0.02 0.04 0.06 0.08 0.10 0.30 0.35 0.40 0.45 0.50 Relative expression GDPD1 B D E F Retinal cone cell Retinal rod cell type A Retinal rod cell type B Retinal rod cell type C Retinal cone cell Retinal rod cell type A Retinal rod cell type B Retinal rod cell type C M/L cones Rods S-cones M/L cones Rods S-cones YPEL2 GDPD1 YPEL2 GDPD1 Figure S8. YPEL2 and GDPD1 expression across tissues and retinal cell types. (A) qPCR expression levels of YPEL2 across healthy human tissues. YPEL2 is ubiquitously expressed in the tissues studied, including retina, with highest expression in brain. (B) GDPD1 is detected at low levels in all tissue types, with higher levels of expression in brain and testis. (C-F) YPEL2 has higher expression in rod photoreceptor cells compared to cones from single cell RNA sequence data. GDPD1 has low expression in all photoreceptor cells. Single cell expression levels and plots were obtained from the Broad Institute Single Cell Portal, and is based on single cell RNA sequencing results of human 26 (C-D) and macaque 27 (E-F) retinal cell types.