Joris van Dongen

111 tSVF acts anti-inflammatory on chondrocytes in vitro Figure 1. Number of viable nucleated cells per mL tissue-like stromal vascular fraction (tSVF). Each dot represents the value of one donor. Table 2. Surface marker expression of tSVF. CD markers Mean ± SD Cell population CD45-; CD90+; CD105+ 48.87% ± 17.59% Mesenchymal stromal cell CD31+; CD34+ 39.55% ± 32.38% Endothelial cell CD45+; CD34- 3.42% ± 3.18% Leukocyte CD34+/-; CD31-; CD146+ 0.23% ± 0.19% Pericyte CD45+; CD34+ 0.32% ± 0.39% Hematopoietic stem cell-like cell CD34bright; CD31-, CD146- 5.95% ± 8.54% Supra-adventitial cell After culture, cells present in tSVF were cultured and characterized based on their ability to form colonies which increased each passage (Fig. 2).Moreover, primary freshly isolated and plastic-adherence expanded tSVF-derived cells were able to differentiate into adipogenic, osteogenic and chondrogenic cell lineages (Fig. 3).