Joris van Dongen

112 Chapter 5 Figure 2. Colony-forming unit assay of tissue-like stromal vascular fraction (tSVF) at P0 (10.000 cells seeded), P1 (1000 cells seeded) and P2 (1000 cells seeded) to examine cell colony formation. The number of violet blue-stained colonies is expressed as a percentage of total seeded nucleated cells. P = passage. Figure 3. Representative bright-field microscope images of tissue-like stromal vascular fraction (tSVF) at P0, P1, P2 and P3 differentiated into the adipogenic (upper, 40x magnification), osteogenic (mid, 10x magnification) and chondrogenic (lower, 10x magnification) lineages as shown by stainings with oil red O, alizarin red and safranin-O, respectively. P = passage.