Joris van Dongen

113 tSVF acts anti-inflammatory on chondrocytes in vitro Cell ratio analysis of chondrocytes and tSVF-derived cells in co-culture Chondrocytes co-cultured with tSVF-derived cells in various ratios i.e. 75% / 25%, 50% / 50% and 25% / 75% (chondrocytes / tSVF-derived cells) resulted in an increase of the ratio chondrocytes / tSVF-derived cells after 21 days compared to the baseline (respectively p<0.05, p<0.01, p<0.01, fig. 4). The total number of chondrocytes was significantly higher when co-cultures of chondrocytes and tSVF-derived cells i.e. 75%/25% and 50%/50% were compared with cultures of chondrocytes alone (p<0.05, fig. 4). Figure 4. Relative lysine demethylase 5D (KDM5D, y-chromosomal) gene expression of male chondrocytes after 0 days and 21 days of culture. Results are expressed as mean ± SD and analysed with an unpaired t-test when different pellets of co-cultures where compared. A paired t-test was used when the same co-culture was analysed at different timepoints. * p = <0.05. ** p = <0.01. Sulphated GAGs analysis No significant difference was observed when mono-cultures of chondrocytes were comparedwith co-cultures of 25%chondrocytes and75%tSVF-derived cells (p>0.05, fig 5). Significantly more sulphated GAGs were present in mono-cultures of chondrocytes in comparison with co-cultures of chondrocytes and tSVF-derived cells of respectively 75% / 25% and 50% / 50% after 28 days (p<0.05, p<0.01, fig. 5).