Joris van Dongen

114 Chapter 5 Figure 5. Sulphated glycosaminoglycan (GAG) deposition normalized for DNA content of pellets from co-cultures of chondrocytes and tSVF-derived cells after 28 days of culture at various ratio´s. Results are expressed as mean ± SD and analysed with an unpaired t-test. * p = <0.05. ** p = <0.01. In Vitro Inflammation Assay A significant downregulation of IL-1 β gene expression was observed in TNF α treated chondrocytes after addition of CM from tSVF-derived cells (p<0.01, fig. 6), but not after the addition of CM from chondrocytes (Fig. 6). COX2 gene expression was significantly downregulated in TNF α treated chondrocytes after addition of CM from chondrocytes and CM from tSVF-derived cells (p<0.05, p<0.01, fig 6). Both IL-1 β and COX2 gene expression was downregulated in TNF α treated chondrocytes after addition of tSVF- derived cells or chondrocytes (p<0.01, p<0.0001, fig. 7), but the gene expressions were more reduced by the addition of tSVF-derived cells compared to chondrocytes (p<0.01, fig 7).