Joris van Dongen

115 tSVF acts anti-inflammatory on chondrocytes in vitro Figure 6. Relative gene expression of interleukin (IL)- β (A) and cyclooxygenase 2 (COX2 (prostaglandin- endoperoxide synthase 2 (PTGS2)) (B) by tumor necrosis factor alpha (TNF α )-stimulated chondrocytes and subsequently treated with conditioned medium (CM) of chondrocytes or tissue-like stromal vascular fraction (tSVF) for 24h. Results are expressed as mean ± SD and analysed with an unpaired t-test. ns = not significant. * p = <0.05. ** p = <0.01. *** p = <0.001. Control = chondrocytes without TNF α treatment. Figure7. Relativegeneexpressionof interleukin(IL)-1 β (A) andcyclooxygenase2 (COX2 (prostaglandin- endoperoxide synthase 2 (PTGS2)) (B) by tumor necrosis factor alpha (TNF α )-stimulated chondrocytes and subsequently treated with chondrocytes or tissue-like stromal vascular fraction (tSVF) cells for 24h. Results are expressed as mean ± SD and analysed with an unpaired t-test. * p = <0.05. ** p = <0.01. *** p = <0.001. **** p = <0.0001. Control = chondrocytes without TNF α treatment.