Joris van Dongen

130 Chapter 6 13. 1% Swine serum. 14. Polyclonal Rabbit anti-Mouse (Dako #P0260, Glostrup, Denmark). 15. Polyclonal Goat anti-Rabbit (Dako #P0448, Glostrup, Denmark). 16. Polyclonal Swine anti-Rabbit (Dako #P0217, Glostrup, Denmark). 17. 3,3’-diaminobenzidine (DAB, Sigma Life Science, St. Louis, MO). 18. Hematoxylin. 19. Mounting solution. Masson’s Trichrome staining of tSVF 1. 4% paraformaldehyde (PFA). 2. 1% triton X-100. 3. Bouin fixative: a. 36 ml of picric acid (saturated). b. 12 ml of 37% formalin. c. 2 ml of acetic acid (glacial). 20. Weighert’s iron hematoxylin: a. 25 ml of stock solution A. b. 25 ml of stock solution B. c. Stock solution A: i. 0.5 g of hematoxylin. ii. 50 ml of 100% alcohol. d. Stock solution B: i. 0.6 g of iron chloride. ii. 49.5 ml of demiwater. iii. 0.5 ml of hydrochloric acid. 5. Biebriech scarlet-acid fuchsine: a. 0.5 g of Biebriech scarlet. b. 0.5 g of acid fuchsine.