Joris van Dongen

137 Isolation of SVF by the FAT procedure NOTES 1. The fractionation of adipose tissue procedure only works when the harvested adipose tissue is separated from all the infiltration fluid and oil by centrifugation at a high speed (960 xg ). When small amounts of infiltration fluid are left behind, none of the adipocytes will be disrupted and therefore no oil will appear after the final centrifugation step . In case of fibrotic adipose tissue is processed by the fractionation of adipose tissue procedure, the fractionator can clog. The fractionator can be cleaned manually with 100% ethanol (Fig. 1). Figure 1. The fractionation of adipose tissue procedure (the FAT procedure). A) The composition of one time centrifuged adipose tissue (1. Adipose tissue, 2. Infiltration fluid) at 960 xg for 2.5 min. In some cases, oil already appears after the first round of centrifugation. B) The composition of adipose tissue (1. Oil (disrupted adipocytes), 2. Tissue SVF, 3. Infiltration fluid) after centrifugation at 960 xg and disruption by means of the fractionator and centrifugation at 960 xg . SVF = stromal vascular fraction.