Joris van Dongen

138 Chapter 6 2. In case some infiltration fluid is left behind, the harvested adipose tissue will turn white when the harvested adipose tissue is pushed forward and backwards through the Luer-to-Luer connector. After the second round of centrifugation, no oil will appear and therefore the stromal vascular fraction cannot be isolated. This is called emulsified adipose tissue; the liquid content is mixed with the adipose tissue content and allows for a better injectable fraction (Fig. 2). Figure 2. Emulsified adipose tissue with the use of the Nanofat procedure. 1) Adipose tissue and 2) Infiltration fluid. 3. Results of the immunohistochemistry images can be analyzed with the use of ImageJ software (freeware, NIH). α -SMA and vWF stainings are measured by drawing a line around the tissue sample of interest and set a threshold to separate positive cells from negative cells. Perilipin A staining can be analysed by manual counting of the positive adipocytes (Fig 3).