Joris van Dongen

139 Isolation of SVF by the FAT procedure 100 μm 100 μm 100 μm 100 μm 50 μm 50 μm 50 μm 50 μm Perilipin Masson’s Trichrome α-SMA vWF tSVF tSVF tSVF tSVF Control Control Control Control Figure 3. Immunohistochemistry staining examples of how tSVF and unprocessed adipose tissue (control) should look like when a perilipin, masson’s trichrome, α -smooth muscle actin ( α -SMA) and von Willebrandfactor (vWF) staining are performed. 4. For a complete characterization of the tSVF, flow cytometry analysis of enzymatic isolated tSVF is advised. Each tube contains all the CD markers used to analyse the preferred cell type present in the tSVF (regardless a positive or negative expression of the CDmarker on the surface of the cell type). It is possible to analyse multiple cell types with the same CD markers in one tube. The maximum number