Joris van Dongen

140 Chapter 6 of CD markers used in a single tube depends on the chosen fluorophore ( e.g. allophycocyanin (APC) and fluorescein isothiocyanate (FITC)). Each CD marker used in a single tube should contain a different fluorophore. 5. Different types of fluorophore-conjugated antibodies can be combined and used to analyze different cell types in the tSVF (Table 1). Table 1 contains a recommended set of CD markers to analyze the most important adipose derived cell types in the tSVF. 13 However, there is no consensus regarding the correct subset of CD markers for each cell type. 3,4,13,20-22 Table 1. The phenotype of the most important adipose derived (CD45 neg ) cell types based on CDmarker expression in freshly isolated tSVF. Cell type CD31 CD34 CD45 CD90 CD105 CD146 Progenitor pericyte Negative Positive Negative Positive Negative Positive Pericyte Negative Negative Negative Positive Negative Positive Supra-adventitial cell Negative Positive Negative Positive Negative Negative Fibroblast Negative Negative Negative Positive Negative Negative Adipose derived stromal cell Negative Positive Negative Positive Low percentage positive Negative Vascular endothelial cell Positive Positive Negative Positive Low percentage positive Positive Endothelial cell Low percentage positive Positive Negative Positive Low percentage positive Positive Low percentage positivity means that there is a small subpopulation that expresses this CD marker. CD45 is used to distinguish between two large populations in tSVF: adipose derived population (CD45 neg ) and blood derived population (CD45 pos ).