Joris van Dongen

162 Chapter 7 Table 2b. Animal studies on lipofilling to reduce pain. Reference Animal model Intervention Follow up Results Huang et al. 2014 Rat Full thickness burn wound of hind paw Treatment: lipofilling (rat adipose tissue) 4 weeks after injury Controls: saline injection or no treatment, and/ or sham burn wound. Behavioral testing for neuropathic pain: paw withdrawal test with mechanical and heat stimuli. Histology of hind paw skin (H&E, MTC) and of spinal cord (microglial activation). All at 4 weeks after lipofilling. Reduction of burn induced allodynia. Improvement of skin histology in burn wound treated with lipofilling: decrease in collagen deposition, increased cellularity. Less microglial activation in spinal cord. All observations for burn wounds treated with lipofilling, compared to saline injection. Huang et al. 2015 Rat Full thickness burn wound of hind paw Treatment: lipofilling (rat adipose tissue) 4 weeks after injury Controls: saline injection and/or sham burn wound Behavioral testing for neuropathic pain: paw withdrawal tests. Assessment of inflammatory markers in hind paw skin (COX-2, iNOS, nNOS) and spinal cord (IL-1β, TNFα, p-IkB and p-NFkB). All at 4 weeks lipofilling. Reduction of burn induced allodynia. Decrease of inflammatory markers in hind paw skin and in spinal cord. Decrease in inflammatory pathway activation (p-IkB and p-NFkB) and in pro-apoptotic pathway activation (p-JNK) in spinal cord. All for burn wounds treated with lipofilling, compared to saline injection. Abbreviations: H&E = hematoxilin and eosin, MTC = Masson’s trichrome, IL-1 β = interleukin 1 beta, COX-2 = cyclo-ogygenase 2, TNF α = tumor necrosis factor alpha, CD31 = cluster of differentiation 31, iNOS = inducible nitric oxide synthase, nNOS = neuronal nitric oxide synthase