Joris van Dongen

232 Chapter 10 of subjects in the experimental group was 51.64 ± 4.24 [range 45 – 58] and 48.93 ± 7.08 [range 38 – 60] in the control group (p>0.05). No difference was seen in baseline platelet count between both groups (p>0.05). Table 2. Age and platelet count at time of surgery of all included subjects in both groups. No significant difference between both groups. PRP = platelet rich plasma; tSVF = tissue stromal vascular fraction. Immunohistochemical and immunocytochemical analysis of tissue- stromal vascular fraction Fractionationof adipose tissue toobtain tSVFwas effective inall patients because almost all adipocytes were disrupted. Reduction of adipocytes resulted in an enrichment of small vessels (determined as mean α -SMA positivity of 21.24% ± 5.02 and mean vWF positivity of 8.95% ± 3.26) and extracellular matrix per remaining 10% volume (Fig. 3, table 3). This study was not designed to focus on any correlation between clinical effect and histological composition of tSVF. Platelet-rich plasma supplemented lipofilling with tissue-stromal vascular fraction does not improve skin elasticity nor reduces TEWL The addition of tSVF showed no difference in total deformation (R7) between both groups at any moment for both evaluation zones except for evaluation zone one at twelve months postoperative (Fig. 4).