Joris van Dongen

255 Facial lipografting on skin quality: a systematic review Table 4. Adipose tissue harvesting and processing characteristics. Author, year Donor site Infiltration solution Harvesting cannula Pressure Processing method Amirkhani et al. 2016 Abdominal Saline solution + epinephrine 0.001% 3 mm - Enzymatic isolation of cSVF with collagenase type 1 Botti et al. 2011 Abdomen, knees, or thigh Saline solution + mepivacaine 0.25% + epinephrine 1:500,000, ratio 1:1 solution:tissue 2 mm two-hole blunt Manual negative pressure <2 cc I1: filtering and washing with the use of strainer and 0.9% NaCl I2: centrifugation at 3000 rpm for 3 min. Charles-de-Sá et al. 2015 Abdominal - 3 mm - I1: non-enzymatic isolation of cSVF by centrifugation at 1286 xg for 3 min. cSVF was mixed with decanted lipografting I2: enzymatic isolation of cSVF and subsequent expansion of ASCs for 5 weeks Covarrubias et al. 2013 Lower hemiabdominal 150 ml of saline solution + adrenaline 0.25g + 20 ml of lidocaine 2% 3 mm - Decantation for 15 min. Liang et al. 2018 Medial and lateral thigh Tumescent technique 3.5 mm polyporous Low negative pressure Centrifugation at 1000 rpm for 2 min., washing with NaCl and subsequently the Nanofat procedure. Nanofat was mixed with PRF. Rigotti et al. 2016 Infraabdominal Lidocaine 0.5% + epinephrine 1:500,000 3 mm three-hole Manual vacuum I1: non-enzymatic isolation of cSVF by centrifugation at 1286 xg for 3 min. cSVF was mixed with decanted lipografting I 2: enzymatic isolation of cSVF and subsequent expansion of ASCs for 4-5 weeks I 3: decanted lipografting was mixed with PRP Song et al. 2017 Abdominal, thighs and buttocks Tumescent technique (not specified) - Low pressure Decantation and wicking