Joris van Dongen

294 Chapter 12 Figure 4. Statistical analyses of changes of the scratch surface area (9h) compared to controls (serum free culture medium and ASC-CMe1) for conditioned medium derived from NAM and DAM hydrogels (n=3) with released factors after 24h, 48h and 96h. NAM = non-diabetic acellular matrix, DAM = diabetic acellular matrix, ASC-CMe1 = one time concentrated (undiluted) adipose derived stromal cell conditioned medium, FBS = fetal bovine serum. *Significant smaller scratch surface area visible after the use of NAM24h, NAM48h, NAM96h as well as DAM48h and DAM96h in comparison with serum free culture medium (p<0.05). Moreover, the use of ASC-CMe1 reduced the scratch area in comparison to serum free culture medium (p<0.05). Significant smaller scratch area visible after the use of ASC-CMe1 in comparison with DAM24h and DAM96h (p<0.05). Results are expressed as mean with standard error of the mean for NAM and DAM hydrogels. Results are expressed as mean with standard deviation for serum free culture medium and ASC-CMe1. Proliferation of PK84 fibroblasts is increased with released factors by CMe-loaded DAM hydrogels Factors released from both NAM and DAM gels promoted proliferation for 96h (Fig. 5). Proliferationwas higher in the DAMgroup as compared to the NAMgroupwith factors being released for 24h and 48h (respectively p<0.01, p<0.05) (Fig. 5). Furthermore, the proliferation was increased in the NAM group with released factors after 24h vs 48h (p<0.001) as well as after 24h vs 96h (p<0.05) and was higher in the DAM group after 24h vs 96h (p<0.01).