Joris van Dongen

295 Adipose ECM hydrogels to release paracrine factors Figure 5. Statistical analyses of fraction of proliferating cells compared to ASC-CMe for conditioned mediumderived fromNAMandDAMhydrogels (n=3)with released factors after24h,48hand96h.NAM = non-diabetic acellular matrix, DAM = diabetic acellular matrix, ASC-CMe1 = one time concentrated (undiluted) adipose derived stromal cell conditionedmedium. *Significantlymore proliferation occurred between NAM24h vs NAM96h and NAM48h vs DAM48h (p<0.05). **Significantly more proliferation occurred between NAM24h vs DAM24h and DAM24h vs DAM96h (p<0.01). ***Significantly more proliferation occurred between NAM24h vs NAM48h (p<0.001). Results are expressed as mean with standard error of the mean for NAM and DAM hydrogels. Similar sprouting ability of HUVEC when treated with released factors by CMe-loaded NAM and DAM hydrogels Sprouting of HUVEC was similar after treatment with factors released from CMe loaded NAM hydrogels compared to DAM hydrogels (p>0.05) (Fig. 6). Moreover, a comparison of the total number of loops between different times i.e. 24h, 48h and 96h of released factors was similar for both types of hydrogels (p>0.05) (Fig. 6). For the NAM groups, released factors from CMe-loaded hydrogels after 24h as well as 96h in comparison with serum free controls showed more loops (p<0.05). Unexpectedly, no significant difference in the group of released factors from CMe loaded NAM hydrogels after 48h compared with serum free medium was found. This is probably caused by large interdonor variation, because the mean number of loops is higher in the group of released factors from CMe loaded NAM hydrogels after 48h. Moreover, these results indicate that CMe loaded hydrogels function as a controlled slow release scaffold of factors that stimulate sprouting even after 96h. For the DAM group,