Joris van Dongen

296 Chapter 12 released factors from CMe-loaded hydrogels after 24h as well as 48h in comparison with serum free culture medium showed more loops (p<0.05). The mean number of loops forASC-CMe1 was comparable with the mean number of loops for the NAM and DAM hydrogels (p>0.05). Figure 6. Statistical analyses of the number of loops of the 6h sprouting assay compared to controls (serum free culture medium and ASC-CMe1) for conditioned medium derived from NAM and DAM hydrogels (=3) with released factors after 24h, 48h and 96h. NAM = non-diabetic acellular matrix, DAM = diabetic acellular matrix, ASC-CMe1 = one time concentrated (undiluted) adipose derived stromal cell conditioned medium, FBS = fetal bovine serum. *Significantly more loops visible after the use of NAM24h, NAM96h, DAM24h as well as DAM48h in comparison with serum free culture medium (p<0.05). **Significantly more loops visible after the use of ASC-CMe1 in comparison with serum free culture medium (p<0.01). Results are expressed as mean with standard error of the mean for NAM and DAM hydrogels. Results are expressed as mean with standard deviation for serum free culture medium and ASC-CMe1. DISCUSSION In this study, we demonstrated that acellular ECM hydrogels from decellularized adipose tissue incubated with factors released by ASC functioned as a controlled slow release scaffold. These ECM hydrogels bound a series of different factors which were released in an incremental fashion for at least 96h and maintained their biological activity. These ECM hydrogels showed to be non-cytotoxic as well. Diabetic origin of