Joris van Dongen

316 Chapter 13 Figure 2. Representative data of CD‐surface marker expression in immortalized ASCs. Immortalized ASC retain cell differentiation capacity to adipocytes, osteoblasts and smooth muscle cells Immortalized ASC successfully differentiated to adipocytes, osteoblasts and smooth muscle cells. This is coherent with the differentiation ability of normal, human isolated ASC (Fig.3). 15 Immortalized ASC retain colony formation capacity The number of colonies in each well consisting of more than 50 cells varied between 3-7%. This is coherent with results reported from colony experiments with normal, human ASC, were the number of colonies should be between 0.1-5%. 15