Joris van Dongen

337 General discussion and future perspectives Another potential indication of using tSVF where inflammation as well as lack of angiogenesis plays a crucial role is diabetic wound healing. Both pathological processes can be positively affected by tSVF. However, some diabetic wounds might be too large to cover entirely with tSVF obtained by the FAT procedure. The low production yield of the FAT procedure is one of the main limitations. Another limitation is the necessity of liposuction prior to performing the FAT procedure. Liposuction is an invasive procedure, which might not always be favorable in patients with a disturbed wound healing. Moreover, isolated autologous adipose tissue might be affected by systemic diseases such as diabetes mellitus. 43-45 For these specific cases, the extracellular matrix based hydrogels with loaded factors from therapeutic cells e.g. ASCs or immortalized pluripotent cells might be ideally as an off the shelve product for several pathologies to replace autologous lipofilling or SVF administration. 46