Joris van Dongen

37 Comparison of SVF isolation procedures: a systematic review Table 4A. Duration, costs and procedure characteristics of intraoperative isolation procedures focusing on cSVF. Name Author Enzymatic/ Non- enzymatic (E/N) Automatic/ Manual/ Semi (A/M/S) Open/ closed (O/C) Isolation details Time (min) Disposable (D)/ reusable (R) cost (Dollar) Volume processed (ml) Capacity (ml) End volume (ml) Maximum volume processed /maximum end volume AIS SundarRaj et al. 2015 E A C Tissue digestion, heating and agitation, three-stage filter system (100 micron, 35 micron, 5 micron porosity) - 133 - - 500 10.8 [4-20] - CHA Aronowitz et al. 2013 E S C Fat bag, adapter, centrifugation, shaking incubator and tissue digestion, cell strainer, cell counter Collagenase 88+/23 D710 80-180 180 - - CYT Aronowitz et al. 2013 E A C Washing (lactated Ringer), tissue digestion and agitation, centrifugation Celase/ Reagent A 90 +/16 D1950 100-180 360 - - Aronowitz et al. 2016 89.4 [85-93] D2400 per 120-360 ml 126 [90- 150] 360 5 [5] 30 Domenis et al. 2015 60 D 250 - Pellet - Lin et al. 2008 90 - - - Pellet - GID-SVF2 Aronowitz et al. 2016 E M C Disposable canister for harvesting, filtration, separation and concentration GIDzyme-50 71.4 [68-75] D1000 per 20-120 ml 53.2 [32-88] 120 7.2 [6-9] 13.3 LIPOK Domenis et al. 2015 E S C 1200 xg centrifugation (with a weight- mesh filter piston), celltibator Liberase (collagenase mixture) - - - - - - Aronowitz et al. 2013 Collagenase 111+/- 18 D530 60-100 100 - - Aronowitz et al. 2016 Time Machine accelerator 120.8 [99-149] D450 per 100 ml 71.4 [40–97] 400 20 [15-25] 3.9 PNC Aronowitz et al. 2013 E M O Centrifugation, shaking incubator, clean bench, HEPA filter, UV-lamp Collagenase 115+/- 13 D460 100-150 400 - - Aronowitz et al. 2016 65.4 [59-74] D250 per 100 ml 105.6 [68-150] 800 12.2 [10.5-15] 10