Joris van Dongen

38 Chapter 2 Table 4A. Continued. Name Author Enzymatic/ Non- enzymatic (E/N) Automatic/ Manual/ Semi (A/M/S) Open/ closed (O/C) Isolation details Time (min) Disposable (D)/ reusable (R) cost (Dollar) Volume processed (ml) Capacity (ml) End volume (ml) Maximum volume processed /maximum end volume SEPAX Güven et al. 2012 E A C Tissue digestion, priming and straining, centrifugation, washing 0.15% NB6 GMP Grade Collagenase 90-120 - 40-400 - Pellet - TGCIS Doi et al. 2012 E A C Tissue digestion, centrifugation, washing, 700 xg centrifugation 0.075% collagenase 65 D 20-60 - Pellet - AIS Automated Isolation System; CHA-station (CHA-Biotech); CYT Celution System Enzymatic (Cytori); GID SVF2 (GID Europe); LIPOK Lipokit System (Medi-khan); PNC Multi station (PNC); SEPAX Sepax (Biosafe); TGCIS Tissue Genesis Cell Isolation System (Tissue Genesis)