Joris van Dongen

45 Comparison of SVF isolation procedures: a systematic review system and the non-enzymatic Residual of emulsified fat and Squeezed fat procedures compared to other intraoperative isolation procedures 66,72,75,76 (Table 7).The percentage of stromal cell population of the SVF isolated by the enzymatic Celution system only differs with 25.2% between two studies 72,74 and 32.8% between two other studies, both evaluated by Aronowitz et al. 70,72 . In general, non-enzymatic procedures yielded same amounts of CD31min/CD34pos stromal cells. The stromal cell population, including pericytes, ASCs and supra-adventitial cells, are the most important cell types in regenerative therapies because of their paracrine effect and multi-lineage differentiation capacity 10,78 . Pericytes defined using other CD markers than to define the stromal cell population are placed separately in the table. The enzymatic Celution system evaluated by Lin et al. resulted in the lowest percentage of pericytes in the SVF (0.8%), but used more than three CD markers to detect pericytes 73 . SundarRaj et al. resulted in a higher percentage (2.0%) of pericytes in SVF obtained by the Automated isolation system, but used only two CD markers to determine the pericyte population and other cell types 69 . The use of multiple CD markers results in a more specific population than the use of less CD markers and so a lower percentage of that specific cell type e.g. pericytes 22 . Bianchi et al. used CD34min/CD146pos/CD90pos to detect the pericyte-like population in the SVF and isolated the highest percentage of pericytes using the non-enzymatic Lipogems procedure as compared to other intraoperative isolation procedures 22 . However, Bianchi et al. mostly used other combinations of CD markers in comparison to other studies 22 . This renders their SVF composition incomparable with SVF compositions obtained by other intraoperative isolation procedures.