Joris van Dongen

75 FAT procedure to obtain SVF for regenerative purposes *** Cell count FAT-SVF Control 0 1.0 × 10 6 2.0 × 10 6 3.0 × 10 6 4.0 × 10 6 Nucleated cells/1ml of adipose tissue Figure 4. Figure 4. Statistic data of number of nucleated cells per 1 ml of FAT-SVF and control samples. Results are presented as mean ± standard deviation (SD). *** FAT-SVF contains significant more nucleated cells in 1 ml as compared to 1 ml of control (p<0.001). FAT-SVF FAT-SVF FAT-SVF Control Control Control A. B. C. Figure 5 . Light microscope and immunofluorescent images of ASC derived from FAT-SVF and control. (A) After Oil Red O staining, lipid droplet accumulation turned red. (B) After Alizarin Red staining, mineralized bone-like nodules turned red. (C) After phalloidin-FITC staining, F-actin turned green. No visual differences between FAT-SVF and control.