Joris van Dongen

76 Chapter 3 Cells cultured from FAT-SVF retain Adipogenic, Osteogenic and Smooth Muscle Cell differentiation capacity All samples of the FAT-SVF and the control showed significant more lipid droplet formation, mineralized bone-like noduli formation and f-actin expression compared to their negative controls (Fig. 6, Supplemental Content). No significant differences were present between the FAT-SVF and the control (Fig. 5). As ASC are known to express F-actin in all cases, all negative controls were slightly positive as anticipated. One sample of the control group detached, most likely due to contraction of F-actin. Cells from FAT-SVF retain colony formation capacity Total colony areas and colony intensities did not differ between ASC derived from the FAT-SVF and controls. This was irrespective of the initial seeding density (P>0.05) (Fig. 6). SVF Control SVF Control 0 2 4 6 8 10 1000 cells seeded 100 cells seeded Colony area covered % SVF Control SVF Control 0 1 2 3 Colony intensity % A. B. Figure 6. Statistic data of (A) total colony area covered and (B) colony intensity of ASC derived from FAT-SVF and control. No significant differences in colony area covered and intensity between ASC derived from FAT-SVF and control when hundred or thousand cells were seeded (P>0.05).