Joris van Dongen

85 FAT procedure to obtain SVF for regenerative purposes Positive control Figure 3. Immunofluorescent images of dead cell control viability. Dead cells turned red after staining with CFDA-SE/PI. Nuclei turned blue after staining with DAPI. FAT-SVF Control 50 µm 50 µm Figure 4. Light microscope images of ASC attached to culture plastic derived from (A) FAT-SVF and (B) control with a 20x original magnification after 8 days. Spindle-shaped fibroblast-like morphology is visible in both groups, no visual differences between both groups.