Joris van Dongen

95 FAT procedure with a disposable one-hole fractionator Fractionation of adipose tissue obtained with either the three-hole fractionator or the one-hole fractionator condenses cells Enzymatic isolation of tSVF obtained by the one-hole fractionator and the three-hole fractionator as well as control lipoaspirate resulted in adipocyte-free cell preparation with a mean viable nuclear cell count of respectively 2.35*10 6 ± 2.98*10 5 , 2.67*10 6 ± 4.63*10 5 and 3.12*10 5 ± 8.99*10 4 (Fig. 2). No quantitative differences were found for ASCs (CD45-; CD90+; CD105+: reusable 41.4% ± 16.5%, disposable 44.9% ± 18.2%), endothelial cells (CD31+; CD34+: reusable 12.0% ± 4.5%, disposable 19.1% ± 2.3%), leukocytes (CD45+; CD34-: reusable 5.3% ± 3.6%, disposable 5.3% ± 3.6%), pericytes (CD34+/-; CD31-; CD146+: reusable 0.3% ± 0.3%, disposable 0.5% ± 0.5%) and hematopoietic stem cell-like cells (CD45+; CD34+: reusable 0.1% ± 0.2%, disposable 0.2% ± 0.3%) after the FAT procedure with either the three-hole fractionator or the one-hole fractionator (Fig. 3). Figure 2. Statistic data of number of viable nucleated cells per 1 mL of tSVF obtained by the reusable fractionator as well as disposable fractionator and control lipoaspirate. Results are presented as mean ± standard deviation. *tSVF obtained by the disposable fractionator contains significant more viable nucleated cells in 1 mL as compared to 1 mL of control (p=0.011).