Joris van Dongen

97 FAT procedure with a disposable one-hole fractionator Fractionation of adipose tissue does not affect colony formation Colony frequency of uncultured cells derived from tSVF by means of the one-hole fractionator and the three-hole fractionator was similar with respectively 1.29% ± 0.038 and 1.29% ± 0.045 (p>0.05) (Fig. 4). Figure 4. Colony frequency of uncultured cells derived from tSVF isolated by the reusable fractionator as well as disposable fractionator. No significant difference between colony frequency of uncultured cells derived from tSVF. P0 = passage 0; tSVF = tissue stromal vascular fraction; the one-hole fractionator = disposable fractionator; the three-hole fractionator = reusable fractionator. DISCUSSION The FAT procedure with the use of the one-hole disposable fractionator showed to be as effective to isolate tSVF with a comparable number of cells and composition of cell types as compared to the former FAT procedure. In our experience, this new system never blocked upon use. The obtained tSVF by means of the one-hole disposable fractionator was composed of condensed vasculature and ECM in comparison with unprocessed adipose tissue. After enzymatic digestion of the tSVF obtained by both types of fractionators, cells were able to attach to tissue culture plastic and form