Joris van Dongen

99 FAT procedure with a disposable one-hole fractionator hole fractionator can occur when lipoaspirate contains substantial amounts of fibrous tissue ( e.g . in secondary donor sites or in male subjects with limited subcutaneous tissue to harvest from). Thus far, multiple in vitro studies have investigated the composition of the isolated tSVF bymeans of the FAT procedure or similar mechanical procedures like the Nanofat procedure. 16,18,32 These studies have shown that tSVF contains increased numbers of ASCs as compared to unprocessed lipoaspirates. 16,32 ASCs are important cell types with a high regenerative potential. As mentioned before, ASCs secrete a plethora of growth factors and cytokines which are able to stimulate important regenerative processes such as matrix remodeling and angiogenesis. 1,17,33 Hence, the obtained tSVF is promising for clinical regenerative purposes, such as scar remodeling and wound healing. However, its clinical effectiveness has not been proven yet as no well-designed randomized double-blind placebo-controlled prospective clinical trials investigating the regenerative potential of tSVFhave been performed.Well-defined prospective trials with the use of mechanical isolated tSVF are therefore warranted. To date, our research group finished the inclusion of two randomized double-blind placebo-controlled clinical trials using tSVF isolated by means of the FAT procedure to improve scarring after breast reductions and to improve skin quality for the aging face. A safe and easy- to-use disposable system, such as the disposable one-hole fractionator as described in this study, might also pave the way towards a comparative study. CONCLUSION The FAT procedure with the one-hole disposable fractionator effectively isolates tSVF with a comparable number of cells and cell composition in comparison with the original described FAT procedure using a three-hole reusable fractionator. However, a disposable one-hole fractionator offers technical and financial advantages over the reusable three-hole fractionator, as it is safer and more user friendly.