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Chapter 5 118 TRPM8 CD64 TRPM8 CD68 TRPM8 Mast Cell Tryptase NPC TRPM8 Blocking peptide Figure S5.3 TRPM8 does not co-label with monocyte/macrophage expressing CD64 and CD68, or with mast cells expressing mast cell tryptase, in human colon tissue. Scale bar=50 μM. Figure S5.4 Assessment of TRPM8 antibody specificity using a) no primary control (NPC) to eliminate non- specific secondary antibody staining and b) blocking peptide to quench TRPM8 binding sites. Scale bar=50 μM.